Coding

Part:BBa_K4949006:Design

Designed by: UManitoba_2023   Group: iGEM23_UManitoba   (2023-10-11)


RPA1511


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 279
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 105
    Illegal NgoMIV site found at 114
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 514
    Illegal NgoMIV site found at 574
    Illegal AgeI site found at 139
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 817


Design Notes

This part has been codon optimized for expression in E.coli.


Source

This part was originally sourced from Rhodopseudomonas palustris

References